LAMP1 recruits to entotic vacuoles prior to LC3. (a) Representative time-lapse widefield microscopy images of entosis in MCF10A cells expressing GFP-hLC3A and LAMP1-RFP. Arrows mark acquisition of fluorescent markers. Scale bar, 15 μm; time, min:s. (b) Analysis of fluorescent marker intensity at the entotic vacuole membrane over time. (c) Quantification of LAMP1-RFP acquisition from 23 entotic vacuoles in relation to GFP-hLC3A (time 0). Data represent mean ± SD. ****, P < 0.0001, unpaired t-test.