Overexpression of Msp1 leads to efficient degradation of mNG-Pex15Δ30 in get1Δget2Δ cells. get1Δget2Δ cells expressing 3xFLAG-mNG-Pex15Δ30 from the GAL1 promoter, with or without overexpression of Msp1 (Msp1 O/E) under the control of the ADH1 promoter, were grown in SCD at 30°C, and then in SCGal for 3 h at 30°C. Normalized relative amounts of 3xFLAG-mNG-Pex15Δ30 were plotted against chase time (right), and the amount of 3xFLAG-mNG-Pex15Δ30 in get3Δ cells, right after the addition of CHX (chase time = 0), relative to that in WT cells without Msp1 overexpression, is shown (center). Values are mean ± SD from three independent experiments. Source data are available for this figure: SourceData FS3.