Localization of Pex15Δ30 to the ER is impaired by defects in Msp1 and the GET pathway. (A–H) WT (A), msp1Δ (B), doa10Δ (C), doa10Δmsp1Δ (D), get3Δ (E), get1Δget2Δ (F), doa10Δget3Δ (G), and doa10Δget1Δget2Δ (H) cells, expressing 3xFLAG-mNG-Pex15Δ30 from the GAL1 promoter, were grown in SCD medium at 30°C and then in SCGal medium for 4 h at 30°C and imaged by fluorescence microscopy. Mitochondria and the ER were labeled with Su9-RFP (upper panels) and ER-mCherry (lower panels), respectively. Single-plane images are shown. Scale bar, 5 μm. DIC, differential interference contrast microscopy. (I and J) Colocalization of 3xFLAG-mNG-Pex15Δ30 with mitochondria (I) and the ER (J) was measured using Pearson’s correlation coefficient between mNG and mitochondria (RFP)/ER(mCherry) signals. Values are mean ± SD (n = 50) from three technical replicates; n represents the number of cells. ***, P < 0.01; ****, P < 0.0001 compared with WT cells by one-way ANOVA with Dunnett’s multiple comparison test.