Figure S8.
Contractomeres move during cell extrusion in mature monolayer. (A) Frames from time-lapse of α-actinin-venus showing contractomeres (yellow circles) glide towards each other to constrict the apical junction during live-cell extrusion (white asterisk). Yellow arrows mark the paths of contractomere movements. Pink and orange lines show shortening of junctions surrounding the extruding cell. Orange circle shows the location of contractomeres after junction constriction is completed. Scale bar is 2 μm. (B) Frames from time-lapse of α-actinin-venus showing contractomeres (blue circles) glide towards each other to constrict the apical junction during apoptotic cell extrusion (orange asterisk). Yellow arrows mark the paths of contractomere movements. Orange circle shows the location of contractomeres after constrictions is completed. Scale bar is 10 μm. (C) Frames from time-lapse of α-actinin-venus showing contractomeres gliding around the extruding cell (white asterisk). Contractomeres circled in blue and orange are immobile while contractomeres next to the extruding cell (circled in yellow, green, and red) are mobile. Movements of contractomeres next to the extruding cell (yellow, red, green circled) shorten the apical junction surrounding the extruding cell (white asterisk) and lengthen the apical junction in the neighboring cells (blue and orange arrows). Scale bar is 5 μm. (D) Frames from time-lapse structured-illumination microscopy of α-actinin-venus showing gliding of contractomeres (circles) to constrict the apical junction (white asterisk). Scale bar is 5 μm. (E) Frames from structured-illumination microscopy of α-actinin-venus showing gliding of contractomeres (circles) to shorten the junction next to the extruding cell (yellow arrows) while lengthen the junction in a neighboring cell (blue arrow). Scale bar is 2 μm. (F) Frames from time-lapse movie of α-actinin-venus showing a cell extrusion event. Merge image shows overlapping junctions except the ones immediately surrounding the extruding cells. Scale bar is 10 μm.

Contractomeres move during cell extrusion in mature monolayer. (A) Frames from time-lapse of α-actinin-venus showing contractomeres (yellow circles) glide towards each other to constrict the apical junction during live-cell extrusion (white asterisk). Yellow arrows mark the paths of contractomere movements. Pink and orange lines show shortening of junctions surrounding the extruding cell. Orange circle shows the location of contractomeres after junction constriction is completed. Scale bar is 2 μm. (B) Frames from time-lapse of α-actinin-venus showing contractomeres (blue circles) glide towards each other to constrict the apical junction during apoptotic cell extrusion (orange asterisk). Yellow arrows mark the paths of contractomere movements. Orange circle shows the location of contractomeres after constrictions is completed. Scale bar is 10 μm. (C) Frames from time-lapse of α-actinin-venus showing contractomeres gliding around the extruding cell (white asterisk). Contractomeres circled in blue and orange are immobile while contractomeres next to the extruding cell (circled in yellow, green, and red) are mobile. Movements of contractomeres next to the extruding cell (yellow, red, green circled) shorten the apical junction surrounding the extruding cell (white asterisk) and lengthen the apical junction in the neighboring cells (blue and orange arrows). Scale bar is 5 μm. (D) Frames from time-lapse structured-illumination microscopy of α-actinin-venus showing gliding of contractomeres (circles) to constrict the apical junction (white asterisk). Scale bar is 5 μm. (E) Frames from structured-illumination microscopy of α-actinin-venus showing gliding of contractomeres (circles) to shorten the junction next to the extruding cell (yellow arrows) while lengthen the junction in a neighboring cell (blue arrow). Scale bar is 2 μm. (F) Frames from time-lapse movie of α-actinin-venus showing a cell extrusion event. Merge image shows overlapping junctions except the ones immediately surrounding the extruding cells. Scale bar is 10 μm.

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