Figure 1.
Superresolution microscopy of two actomyosin structures at the apical junction. (A) Apical stress fiber has an alternating myosin IIB and synaptopodin pattern and lies parallel to the E-cadherin junction. Orange arrowheads point to the end of an apical stress fiber marked by E-cadherin. White arrowheads point to junctional regions where synaptopodin is in proximity to E-cadherin. White long arrow shows the stretch of junction used for the x-axis of the graph. Scale bar is 500 nm. (B) Apical stress fiber is inserted head-on to E-cadherin junction, marked by an orange arrowhead. Synaptopodin overlaps with E-cadherin at the insertion point of the stress fiber, in white circle. White arrowheads mark the sites where synaptopodin is in proximity to or overlapping with E-cadherin. Scale bar is 500 nm. (C) Disassembly of apical stress fibers in maturing junction leads to loss of alternating pattern of synaptopodin and myosin IIB. Disassembly of apical stress fiber coincides with formation of type II actomyosin structures containing myosin IIB, synaptopodin, and α-actinin-4, marked by white arrowheads on the left panel and blue arrows in graph. White long arrow on the left panel shows the stretch of junction used for the x-axis of the graph. White circles in the right panels show type II structures and white arrowheads in the right panels mark the colocalization of myosin IIB, synaptopodin, α-actinin-4. Scale bars are 1 μm. (D) Apical stress fibers disappear upon junction maturation whereas type II actomyosin structures are prominent at mature apical junctions. Actin accumulates at type II actomyosin structure, circled in white, colocalizing with myosin IIB, synaptopodin (Synpo), and α-actinin-4, as marked by white arrowheads. Graph shows the absence of apical stress fiber and the presence type II structure, named contractomere. White long arrow shows the stretch of the junction used for x-axis of the graph. Scale bar is 1 μm. (E) Two actomyosin structures at the apical junction of MDCK cells. Apical stress fibers are labeled as Type I actomyosin structures with alternating synaptopodin and myosin IIB organization. In maturing junction, Type I apical stress fiber coexists with Type II contractomeres with overlapping synaptopodin (Synpo-DYK) and myosin IIB. White arrows point to synaptopodin colocalizing with myosin IIB. Basal stress fibers have alternating synaptopodin and myosin IIB organization, same as Type I apical stress fibers. Scale bars are 1 μm.

Superresolution microscopy of two actomyosin structures at the apical junction. (A) Apical stress fiber has an alternating myosin IIB and synaptopodin pattern and lies parallel to the E-cadherin junction. Orange arrowheads point to the end of an apical stress fiber marked by E-cadherin. White arrowheads point to junctional regions where synaptopodin is in proximity to E-cadherin. White long arrow shows the stretch of junction used for the x-axis of the graph. Scale bar is 500 nm. (B) Apical stress fiber is inserted head-on to E-cadherin junction, marked by an orange arrowhead. Synaptopodin overlaps with E-cadherin at the insertion point of the stress fiber, in white circle. White arrowheads mark the sites where synaptopodin is in proximity to or overlapping with E-cadherin. Scale bar is 500 nm. (C) Disassembly of apical stress fibers in maturing junction leads to loss of alternating pattern of synaptopodin and myosin IIB. Disassembly of apical stress fiber coincides with formation of type II actomyosin structures containing myosin IIB, synaptopodin, and α-actinin-4, marked by white arrowheads on the left panel and blue arrows in graph. White long arrow on the left panel shows the stretch of junction used for the x-axis of the graph. White circles in the right panels show type II structures and white arrowheads in the right panels mark the colocalization of myosin IIB, synaptopodin, α-actinin-4. Scale bars are 1 μm. (D) Apical stress fibers disappear upon junction maturation whereas type II actomyosin structures are prominent at mature apical junctions. Actin accumulates at type II actomyosin structure, circled in white, colocalizing with myosin IIB, synaptopodin (Synpo), and α-actinin-4, as marked by white arrowheads. Graph shows the absence of apical stress fiber and the presence type II structure, named contractomere. White long arrow shows the stretch of the junction used for x-axis of the graph. Scale bar is 1 μm. (E) Two actomyosin structures at the apical junction of MDCK cells. Apical stress fibers are labeled as Type I actomyosin structures with alternating synaptopodin and myosin IIB organization. In maturing junction, Type I apical stress fiber coexists with Type II contractomeres with overlapping synaptopodin (Synpo-DYK) and myosin IIB. White arrows point to synaptopodin colocalizing with myosin IIB. Basal stress fibers have alternating synaptopodin and myosin IIB organization, same as Type I apical stress fibers. Scale bars are 1 μm.

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