Synaptopodin is localized to junctions and stress fibers of epithelial and endothelial cells. (A) Synaptopodin is encoded by three exons, resulting in four splice variants. We have raised antibodies to each spliced region to characterize the expression of synaptopodin isoforms in mammalian tissues and cells. Western blot of cell lysates using antibodies against region encoded by exon 2, thus recognizing all synaptopodin isoforms. Table summarizes result from Western blot using our newly generated antibodies (see Materials and methods). (B) Immunofluorescence of MDCK kidney tubule epithelial cells, C2bbE2 intestinal epithelial cells, HUVEC endothelial cells. Scale bars are 2 μm.