The PxL3 motif is required for Cdc14-mediated Atg13 dephosphorylation. (A) IB of extracts from synchronized meiotic cells with indicated antibodies. Cell with ATG13 variants simultaneously expresses mCherry-GFP (pZD: mCherry-GFP) and NDT80, induced by 1 µM β-estradiol. 6SA restores mCherry-GFP processing in PxL3m to the WT level. (B) Meiotic kinetics in nonsynchronized meiotic cells expressing ATG13 variants, as indicated, by IF of Tub1. Symbols mark M-II peak position in WT (red dashed line), delayed M-II (red arrow), faster M-II (black arrow), and M-II similar to WT (gray arrow). (C) Sporulation was triggered in nonsynchronized meiotic cells expressing indicated ATG13 variants, which were carried by pRS303 vector in atg13Δ strain. Shown are the percentage of cells sporulated after 48 h in SPM (n ≥ 300 cells; t test; **, P ≤ 0.01; ***, P ≤ 0.001; N.S., not significant). (D) Signal transduction model of Cdc14 stimulating autophagy spatiotemporally. At anaphase I and II, active Cdc14 relocates from nucleolus to cytosol and dephosphorylates Atg13 at PAS to activate atg1 and hence autophagy. Source data are available for this figure: SourceData F8.