Autophagy initiation is upregulated in anaphase I and II. (A and B) FM analysis of Atg1-mNG cells under synchronized meiosis, prophase I arrest, mitotic log phase, starvation (1 h, SD-N), and extended starvation (12/14 h, SD-N). Shown are representative FM images (A) and percentage of cells with Atg1-mNG puncta (B; n ≥ 300 cells at each time point from three replicate experiments; t test). (C) Meiotic kinetics of experiment A determined by IF of Tub1, showing upregulated Atg1-mNG puncta formation at onset of A-I (2 h), A-II (4 h), and post–A-II (6 h). (D–F) FM analysis of mNG-Atg8 puncta and Tub1-mCherry in live cells during synchronized meiosis. Meiotic stages were identified by the specific Tub1-mCherry distribution pattern depicted on top. (D) Representative images from composite of five planes (Z-step size, 0.5 µm). Scale bar, 5 µm. (E and F) Quantitation of C (n ≥ 300 cells at each meiotic stage from three replicate experiments, t test; **, P ≤ 0.01; ***, P ≤ 0.001). Red line in F indicates the median.