Figure S1.
Autophagy in meiotic cells, starved cells, and meiotic ndt80Δ cells. (A) IB of cell lysates with indicated antibodies showing GFP-Atg8 processing. Whole cell extracts derived from cells during nonsynchronized meiosis were treated as diagrammed in Fig. 1 A and analyzed by IB with indicated antibodies. GFP-Atg8 expression was induced upon SPM incubation (t = 0 h). (B) Top: IB (α-Ndt80) showing Ndt80 level in the same samples prepared as in A. Bottom: Proteins transferred to the blotting membrane were stained with Ponceau S before IB. (C and D) IB of whole cell extracts derived from cells under starvation (SD-N) with indicated antibodies. (C) Top: Schematic of GFP-Atg8 induction and cell collection. Bottom: IB images. (D) Quantification of IB intensity for GFP and GFP-Atg8 (normalized by Hxk1 IB intensity) over time course. The window of time matching meiotic divisions in Fig. 1 B (9–16 h) is marked by red box (dashed). GFP-Atg8 expression was induced by 1 µM β-estradiol upon SD-N starvation (t = 0 h). (E and F) IB of whole cell extracts derived from ndt80Δ cells under meiosis/sporulation condition (SPM) with indicated antibodies. (E) Top: Schematic of GFP-Atg8 induction and cell collection. Bottom: IB images. (F) Quantification of IB (α-GFP) intensity for GFP and GFP-Atg8 (normalized by Hxk1 IB intensity) over time course. The window of time matching meiotic divisions in Fig. 1 B (9–16 h) is marked by red box (dashed). GFP-Atg8 expression was induced by 1 µM β-estradiol upon SPM incubation (t = 0 h). (G) Time-lapse FM analysis of Cdc14-mTFP1 foci intensity during quiescence (red) and meiosis (blue). Shown are Cdc14-mTFP1 foci signal (line) with the standard deviations (shade). A.U., arbitrary unit. Source data are available for this figure: SourceData FS1.

Autophagy in meiotic cells, starved cells, and meiotic ndt80Δ cells. (A) IB of cell lysates with indicated antibodies showing GFP-Atg8 processing. Whole cell extracts derived from cells during nonsynchronized meiosis were treated as diagrammed in Fig. 1 A and analyzed by IB with indicated antibodies. GFP-Atg8 expression was induced upon SPM incubation (t = 0 h). (B) Top: IB (α-Ndt80) showing Ndt80 level in the same samples prepared as in A. Bottom: Proteins transferred to the blotting membrane were stained with Ponceau S before IB. (C and D) IB of whole cell extracts derived from cells under starvation (SD-N) with indicated antibodies. (C) Top: Schematic of GFP-Atg8 induction and cell collection. Bottom: IB images. (D) Quantification of IB intensity for GFP and GFP-Atg8 (normalized by Hxk1 IB intensity) over time course. The window of time matching meiotic divisions in Fig. 1 B (9–16 h) is marked by red box (dashed). GFP-Atg8 expression was induced by 1 µM β-estradiol upon SD-N starvation (t = 0 h). (E and F) IB of whole cell extracts derived from ndt80Δ cells under meiosis/sporulation condition (SPM) with indicated antibodies. (E) Top: Schematic of GFP-Atg8 induction and cell collection. Bottom: IB images. (F) Quantification of IB (α-GFP) intensity for GFP and GFP-Atg8 (normalized by Hxk1 IB intensity) over time course. The window of time matching meiotic divisions in Fig. 1 B (9–16 h) is marked by red box (dashed). GFP-Atg8 expression was induced by 1 µM β-estradiol upon SPM incubation (t = 0 h). (G) Time-lapse FM analysis of Cdc14-mTFP1 foci intensity during quiescence (red) and meiosis (blue). Shown are Cdc14-mTFP1 foci signal (line) with the standard deviations (shade). A.U., arbitrary unit. Source data are available for this figure: SourceData FS1.

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