pyd affects SCAR localization to vertices. (A and B) pyd mutant clones stained for SCAR (A) during 26 h APF and after pruning (32 h APF; B). SCAR levels increase in pyd clones compared with WT cells. (C) Paired comparisons of relative levels of SCAR at vertices of LCs in mutant compared with WT cells in multiple clones (left) and within a single clone (right). Wilcoxon test, n = 8 clones and adjacent WT regions from 3 eyes, 10–30 vertices per region, P = 0.0078. Single clone: unpaired t test, n = 30 vertices per region, P < 0.0001.