Pyd::GFP pulse analyses. (Corresponds to Fig. 7.) (A) Trace of Pyd::GFP levels relative to contact length in a single LC–LC contact at 28 h APF. (B) Correlation between Pyd::GFP levels and contact length in A. (C) Schematics of contact length fluctuations and the scheme for measuring Pyd::GFP levels relative to the fluctuations in contact length. (D) Paired comparisons of relative levels of Pyd::GFP at the different phases of contact pulsing in WT eyes. Maximal length: n = 12, P = 0.2620; minimal length: n = 22, P = 0.0391; contraction: n = 26, P < 0.0001; expansion: n = 22, P = 0.0006. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. All statistics performed are Wilcoxon tests.