F-actin assembly and tension enhance Sdk localization to vertices. (Corresponds to Figs. 5 and 6.) (A and B) Dissected pupal eyes expressing Sdk::GFP were incubated in M3 medium and treated with DMSO (A; control) or the actin-monomer-binding toxin Latrunculin A in DMSO (B) and stained for E-cad (blue) and F-actin (red). (C and D) Dissected wing imaginal discs expressing Sdk::GFP were incubated in M3 medium and treated with deionized water (C; control) or the Rok inhibitor Y-27632 (D) and stained for E-cad (blue). Scale bar, 5 μm in A–D.