Sdk affects F-actin and Rok dynamics at LC–LC contacts. (Refers to Table S2 and Video 3.) (A–C) Actin and Rok dynamics in WT (A), sdk^Δ15 mutant (B), and GMR>HA::Sdk eyes (C). Top: Snapshots from a time-lapse video. Bottom: Kymographs of a cell contact. Boxes correspond to time points shown in top panel. (D–F) Time-shifted Pearson correlation plots for contact length versus F-actin. In this and subsequent plots, black line is the mean correlation, gray band the SD. Length positively correlates with F-actin in WT (D) at a shift of −4 min, and in sdk^Δ15 (E) at a shift of −3 min, but no correlation is detected in GMR>HA::Sdk (F). (G–I) Pearson correlation plots for contact length versus Rok. Length negatively correlates with Rok in WT (G) at a shift of −2 min and in sdk^Δ15 (H) at a shift of −1 min. (I) GMR>HA::Sdk: length has a weak negative correlation with Rok at a shift of −1 min. (J and K) Levels of F-actin (J) and Rok (K) in contacts with maximal length relative to contacts with minimal length in WT, sdk mutants, and GMR>HA::Sdk eyes. Kruskal–Wallis test of F-actin ratios, P = 0.0090. Dunn’s multiple comparisons test: WT versus sdk^Δ15, P = 0.0327; WT versus GMR>HA::Sdk, P = 0.0146. Rok ratios, P = 0.0435; WT versus GMR>HA::Sdk, P = 0.0293; WT versus sdk^Δ15, P > 0.9999. Scale bar, 5 μm.