Examination of co-localization of SS-GFP-Lys containing vesicles with various organelle marker proteins (related to Fig. 3 and Fig. 4 ). (A) EpH4 cells expressing SS-GFP-Lys were transfected with mScarlet-Giantin, mCherry-TGNP, mCherry-Sec61β, mRFP-LAMP1, mCherry-Rab5, mCherry-Rab7A and mCherry-Mito-7 expression vectors. Scale bar, 5 μm. (B) EpH4 cells expressing SS-GFP-Lys and the proteins as described in A were treated with 250 nM Torin-1 for 24 h. Scale bar, 5 μm. (C) Treatment with inhibitors of mammalian target of rapamycin signal pathway increased the number of SS-GFP-Lys positive vesicles. Low magnification images of Fig. 4 A. Scale bar, 10 µm.