TRIM1 decreases LRRK2’s activation by Rab29. (a) Immunoblot of LRRK2 phosphorylation at Ser1292, Rab29 phosphorylation at Thr71, and Rab10 phosphorylation at Thr73 in the presence and absence of TRIM1 for WT LRRK2 and LRRK2-PD mutant R1441G. (b) Quantification of LRRK2 autophosphorylation in panel a. (c) Quantification of Rab29 Thr71 phosphorylation in panel a. (d) Quantification of Rab10 Thr73 phosphorylation in panel a. Quantifications show the mean value from three to four independent replicates, with error bars showing SEM. Significance testing for b–d was performed using Kruskal–Wallis with post hoc Dunn test and Bonferroni correction. Source data are available for this figure: SourceData F7.