TRIM1 binds LRRK2-RL to cause LRRK2 microtubule localization. (a) Schematic of GFP-LRRK2 constructs (above) with corresponding live-cell microscopy in the presence of mCherry-TRIM1 in H1299 cells (below). Each panel shows only fluorescence at 488 nm (GFP) to illustrate the subcellular localization of each GFP-LRRK2 construct in the presence of mCherry-TRIM1, which is always localized to the microtubule network. MT, microtubule. (b) Live-cell confocal microscopy of mCherry-LRRK2 in the presence of EBFP2-14-3-3 in H1299 cells. (c) Live-cell confocal microscopy of mCherry-LRRK2 in the presence of EBFP2-14-3-3 and GFP-TRIM1 showing individual EBFP2, GFP, and mCherry channels from Fig. 6 a. In all panels, scale bar = 10 μM..