Figure 6. Evidence of Kap compensation... | Rockefeller University Press

Figure 6.

$Evidence of Kap compensation at the NPC. (A) A significant fraction of Kapβ1 is depleted from the NE following Kapβ1 silencing. This correlates with (1) an increased enrichment of CRM1 at the NE and (2) increases in both the NE/C and NE/N ratios of Kapβ1 and CRM1, respectively. This suggests that the cytoplasmic pool of Kapβ1, together with the nuclear pool of CRM1, has been recruited to compensate for the depleted Kapβ1 molecules at the NPCs. (B) A small fraction of CRM1 is reduced at the NE following CRM1 silencing. This correlates with (1) an enriched pool of Kapβ1 at the NE that is relatively unchanged, (2) no change to the NE/N ratio of CRM1, and (3) a slight increase in the NE/C ratio of Kapβ1. This suggests that only a small fraction of Kapβ1 is being recruited from the cytoplasm to compensate for depleted CRM1 molecules at the NPCs. For explanation, see the main text. Statistical analysis was performed using the Kruskal-Wallis test. P adjusted values were calculated using the Benjamini-Hochberg procedure (****, P = 0.0001; **, P = 0.0021; * P = 0.0332; ns = 0.1). Scale bars, 10 µm.$

Evidence of Kap compensation at the NPC. (A) A significant fraction of Kapβ1 is depleted from the NE following Kapβ1 silencing. This correlates with (1) an increased enrichment of CRM1 at the NE and (2) increases in both the NE/C and NE/N ratios of Kapβ1 and CRM1, respectively. This suggests that the cytoplasmic pool of Kapβ1, together with the nuclear pool of CRM1, has been recruited to compensate for the depleted Kapβ1 molecules at the NPCs. (B) A small fraction of CRM1 is reduced at the NE following CRM1 silencing. This correlates with (1) an enriched pool of Kapβ1 at the NE that is relatively unchanged, (2) no change to the NE/N ratio of CRM1, and (3) a slight increase in the NE/C ratio of Kapβ1. This suggests that only a small fraction of Kapβ1 is being recruited from the cytoplasm to compensate for depleted CRM1 molecules at the NPCs. For explanation, see the main text. Statistical analysis was performed using the Kruskal-Wallis test. P adjusted values were calculated using the Benjamini-Hochberg procedure (****, P = 0.0001; **, P = 0.0021; * P = 0.0332; ns = 0.1). Scale bars, 10 µm.