Actin organization in cells with defective late endosomes. (A–H) Cells expressing CD4::mIFP and Utr::GFP under btl-gal4. (A–F) Control (A–A’’), cell expressing Vha100^R755A (V100^R755A; C–C’’), wash knockdown (E–E’’). Regions marked in A, C, and E are magnified and shown over four time points in B and B’, D and D’, and F and F’, respectively. See also Video 5. (G) Mean Utr::GFP fluorescence intensity in CD4 vesicles over time. Values were normalized to fluorescence intensity at the tube to adjust for variations in the expression of the Utr::GFP reporter. Box plot represents median, IQR, and IQR*1.5 below and above the IQR. Number of vesicles analyzed: control, n = 23 from nine cells; Vha100^R755A, n = 19 from eight cells; wash-IR, n = 20 from nine cells; sn-IR,n = 10 from five cells; btsz-IR, n = 20 from eight cells. Values at the bottom are significance between normal versus misguided cells within each genotype, assessed by t test. Ventral is down.