Actin enrichment around late endosomes. (A and B) Terminal cells expressing Rab7::YFP and stained with phalloidin and a CBP fused to Alexa647. (A–A’’’) Channels shown in pairwise combinations. Arrowheads point to the contact between Rab7::YFP rings, actin, and the tip of the subcellular tube. (B) Close-ups of the same cells as in A, but shown as single Z-planes. (B') Phalloidin. (B'') Rab7::YFP. (C) Correlation between pixel fluorescence intensities in the phalloidin and YRab7 channels, in maximum-intensity projections (as shown in A panels) versus single Z-planes (as shown in B panels). Significance was assessed using two-tailed paired t test, n = 8. Ventral is down.