Actin organization at late endosomes. (A and B) Terminal cell expressing Rab7::YFP and stained with phalloidin and a CBP fused to Alexa647. (A–A’’’) Channels shown in pairwise combinations. (B–B’’’) The region marked in A is magnified in B–B’’’. Arrowheads point to the contact between a Rab7::YFP ring, actin, and the tip of the subcellular tube. (C–F) Terminal cells expressing CD4::mIFP and Utr::GFP under btl-gal4. (C and D) High temporal–resolution imaging. The square shown in C–C’’ is magnified in D and D’ over four different time points (see also Video 3). Arrowheads point to CD4 vesicles. (E and F) High spatial–resolution imaging. Overview of the cell (E–E’’) and four successive time points (F–F’’) of the tip of the same cell. Arrowheads point to actin structures ahead of the subcellular tube.