Figure 8.
ORP5 and seipin biochemically interact in lysates of HeLa cell. (A) Western blot analysis of ORP5A or ORP5B immunoprecipitated (IP) from lysates of HeLa cells co-expressing EGFP or seipin-EGFP with HA-ORP5A or HA-ORP5B, using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5B). (B) Western blot analysis of co-IP products from HeLa cells co-expressing EGFP or seipin-EGFP with ORP5A or ORP5∆ORD using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5∆ORD). (C) Relative quantification of ORP5A or ORP5∆ORD co-immunoprecipitated with seipin-EGFP or EGFP alone. (D) Western blot analysis of co-IP products from HeLa cells co-expressing EGFP or seipin-EGFP with HA-ORP5A or HA-ORP5∆TM, using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5∆TM). (E) Relative quantification of ORP5A or HA-ORP5∆TM co-immunoprecipitated with seipin-EGFP or EGFP alone.

ORP5 and seipin biochemically interact in lysates of HeLa cell. (A) Western blot analysis of ORP5A or ORP5B immunoprecipitated (IP) from lysates of HeLa cells co-expressing EGFP or seipin-EGFP with HA-ORP5A or HA-ORP5B, using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5B). (B) Western blot analysis of co-IP products from HeLa cells co-expressing EGFP or seipin-EGFP with ORP5A or ORP5∆ORD using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5∆ORD). (C) Relative quantification of ORP5A or ORP5∆ORD co-immunoprecipitated with seipin-EGFP or EGFP alone. (D) Western blot analysis of co-IP products from HeLa cells co-expressing EGFP or seipin-EGFP with HA-ORP5A or HA-ORP5∆TM, using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5∆TM). (E) Relative quantification of ORP5A or HA-ORP5∆TM co-immunoprecipitated with seipin-EGFP or EGFP alone.

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