Figure S5.
Increased supernumerary centrioles at low temperature is only observed in plp mutant NBs. Fixed NBs stained for Asterless (Asl, magenta) to label centrioles, Phalloidin (green), and DAPI (blue). Centrioles were counted by counting the number of Asl positive puncta in each cell. Raising flies in lower temperatures only increased in the percentage of NBs carrying more than two Asl puncta in plp mutants (plp2172/plpdf). 18°C: Control = 0.7% ± 0.8 (n = 4 brains, 230 NBs), plp = 8.8% ± 3.6 (n = 4 brains, 330 NBs), khc = 1.3% ± 2.7 (n = 4 brains 235 NBs). 15°C: Control = 1.9% ± 1.5 (n = 4 brains, 205 NBs), plp = 52% ± 6.7 (n = 4 brains, 161 NBs), khc = 1.6% ± 2.3 (n = 4 brains, 197 NBs). Data = mean ± SD. Scale bars: 5 µm.

Increased supernumerary centrioles at low temperature is only observed in plp mutant NBs. Fixed NBs stained for Asterless (Asl, magenta) to label centrioles, Phalloidin (green), and DAPI (blue). Centrioles were counted by counting the number of Asl positive puncta in each cell. Raising flies in lower temperatures only increased in the percentage of NBs carrying more than two Asl puncta in plp mutants (plp2172/plpdf). 18°C: Control = 0.7% ± 0.8 (n = 4 brains, 230 NBs), plp = 8.8% ± 3.6 (n = 4 brains, 330 NBs), khc = 1.3% ± 2.7 (n = 4 brains 235 NBs). 15°C: Control = 1.9% ± 1.5 (n = 4 brains, 205 NBs), plp = 52% ± 6.7 (n = 4 brains, 161 NBs), khc = 1.6% ± 2.3 (n = 4 brains, 197 NBs). Data = mean ± SD. Scale bars: 5 µm.

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