Interphase motility is dependent on intact MT networks. (A) Peripodial cells following 1 h ice treatment followed by recovery in DMSO or 50 µM Colcemid. Note: no visible MTs remaining in the Colcemid treated wing disc. (B) 10-min time projection of centriole movement (colored tracks). Centrioles are not motile following ice treatment and Colcemid recovery. (C and D) Colchicine treatment does not destroy the pre-existing MT network (C) but does block MT dynamics revealed by EB-1 localization (D). (E) 10-min time projections of centriole movement (colored tracks). Centrioles remain highly motile following Colchicine treatment. (F) Quantification of instantaneous velocity in the indicated conditions (ice + DMSO: 117 ± 8.1 n = 6 wing discs, 95 centrioles. ice + Colcemid: 20.7 ± 2.8, n = 7 wing discs, 158 centrioles. DMSO: 115.5 ± 7.4, n = 4 wing discs, 78 centrioles. Colchicine: 77.9 ± 13.2, n = 7 wing discs, 109 centrioles). Data = mean ± SD, P values derived from unpaired t test. (G) Average mean squared displacement of centrioles. Scale bars: 5 µm. Time stamp: mm:ss.