α-Catenin flows from the cell edge with actin and vinculin. (A and A′) Frame from a video of a cell expressing GFP-α-catenin and td-Tomato-Tractin showing colocalization of actin and α-catenin at the edge; and kymographs taken from the yellow line (A′). The chart on the right shows quantifications of the flow rates of α-catenin and actin in adhesions (n = 18 adhesions in each case). (B and B′) Average of six frames (equivalent to 2 min) from a video of a cell expressing GFP-α-catenin and mCherry-vinculin showing colocalization at the edge as well as in mature adhesions (arrow); and kymographs taken from the yellow line (B′). The chart on the right shows quantifications of the flow rates of α-catenin and vinculin in adhesions (n = 24 adhesions in each case). Statistical analysis for the flow rates was performed with two-tailed unpaired t test followed by Welch’s correction.