Cactin regulates aPKC and Crb localizations via its spliceosome function. (A–F) Representative images of egg chambers expressing RNAi against the indicated spliceosome complex components. Anti-aPKC and anti-Arm co-staining c306-Gal4 border cell clusters together with (A) control (crossed to w1118), (B) UAS-cactin-RNAi (as a positive control), (C) UAS-tsu-RNAi, (D) UAS-SmB-RNAi, (E) UAS-SmD3-RNAi, or (F) UAS-noi-RNAi. Yellow arrowheads indicate the aPKC accumulation in the apical junctions. (A′–F′) Images show the single channel of anti-aPKC in A–F. Scale bars, 20 μm. (G) Quantification of delamination defects in stage 10 egg chambers. c306-Gal4 crosses to w1118 used as the negative control and c306-Gal4 driven UAS-cactin-RNAi used as the positive control. (H) mRNA fold change of aPKC isoforms assessed by qPCR. Each dot represents an independent biological replicate. (I) Proportion of mRNA levels of different aPKC isoforms. mRNA levels of aPKC isoforms were normalized to aPKC_com, which detected all aPKC isoforms. Each dot indicates an independent biological replicate in the qRT-PCR. (J) mRNA fold change of Crb isoforms. (K) Proportion of mRNA levels of different Crb isoforms. (L) mRNA fold change of Rab5/7/11. Controls are shown in black bars; cactin-RNAis are shown in red bars. Asterisks indicate P < 0.05.