Figure S5.
Cactin regulates aPKC and Crb localizations via its spliceosome function. Related to Figs. 5 and 6. (A–B″) Images of anti-aPKC staining of the posterior follicle cells with c306-Gal4 together with (A–A″) UAS-SmD3-RNAi or (B–B″) UAS-SmB-RNAi. (A′ and B′) DNA single channel. (A″ and B″) aPKC single channel. Scale bars, 20 μm. (C) Schematic of aPKC isoforms and primers designs for qRT-PCR. Orange rectangles represent the protein coding regions in exons. Gray rectangles represent the UTR regions in exons. Black lines between orange and gray rectangles represent introns. Red arrows represent the primers designed for qRT-PCR. (D) Gel electrophoresis image showing RT-PCR results of aPKC isoforms. (E) Schematic of Sec23 isoforms. (F) Schematic of Sec24CD isoforms. Note that C, E, and F are snapshots from the flybase website (https://www.flybase.org). Source data are available for this figure: SourceData FS5.

Cactin regulates aPKC and Crb localizations via its spliceosome function. Related to Figs. 5 and 6. (A–B″) Images of anti-aPKC staining of the posterior follicle cells with c306-Gal4 together with (A–A″) UAS-SmD3-RNAi or (B–B″) UAS-SmB-RNAi. (A′ and B′) DNA single channel. (A″ and B″) aPKC single channel. Scale bars, 20 μm. (C) Schematic of aPKC isoforms and primers designs for qRT-PCR. Orange rectangles represent the protein coding regions in exons. Gray rectangles represent the UTR regions in exons. Black lines between orange and gray rectangles represent introns. Red arrows represent the primers designed for qRT-PCR. (D) Gel electrophoresis image showing RT-PCR results of aPKC isoforms. (E) Schematic of Sec23 isoforms. (F) Schematic of Sec24CD isoforms. Note that C, E, and F are snapshots from the flybase website (https://www.flybase.org). Source data are available for this figure: SourceData FS5.

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