Figure 4.
Delamination is partially rescued by reducing apical aPKC in cactin-RNAi–expressing cells. (A–D) Images of anti-aPKC staining border cell clusters with c306-Gal4 driven UAS-cactin-RNAi with (A) control (crossed to w1118), (B) UAS-YFP-Rab5, (C) UAS-YFP-Rab7, or (D) UAS-YFP-Rab11. Yellow arrowheads indicate the apical junctions in the border cell cluster. (A′–D′) Images show the single channel of aPKC in A–D. (E and F) Images of anti-aPKC and Arm co-staining border cell clusters of c306-Gal4 driven UAS-cactin-RNAi together with (E) control (cross to w1118) or (F) aPKC null mutant heterozygote. Yellow arrowheads indicate the apical junctions in the border cell cluster. (E′ and F′) Images show the single channel of aPKC in E and F. (G) Schematic of Grab-FP system in which an anti-GFP nanobody is tethered either apically or basolaterally and thereby relocalizes GFP-tagged target proteins to those domains. (H and I) Images of posterior follicle cells in egg chambers of c306-Gal4 combined with EGFP-aPKC together with (H) UAS-GrabFP-Apical or (I) UAS-GrabFP-Basal. Yellow arrowheads indicate the basolateral junctions in the follicle cells. (H′ and I′) Images show the single channel of EGFP-aPKC in H and I. (J and K) Images of anti-GFP and Arm co-staining border cell clusters of c306-Gal4 driven UAS-cactin-RNAi, UAS-GrabFP-Basal together with (J) control (cross to w1118) or (K) EGFP-aPKC heterozygote. (L and M) Images of anti-GFP and Arm co-staining border cell clusters of c306-Gal4 driven UAS-GrabFP-Apical together with (L) UAS-cactin-RNAi or (M) EGFP-aPKC heterozygote. Yellow arrowheads indicate the apical junctions in the border cell cluster. (J′–M′) Images show the single channel of anti-GFP in J–M. (N) Quantification of delamination defects in stage 10 egg chambers.

Delamination is partially rescued by reducing apical aPKC in cactin-RNAi–expressing cells. (A–D) Images of anti-aPKC staining border cell clusters with c306-Gal4 driven UAS-cactin-RNAi with (A) control (crossed to w1118), (B) UAS-YFP-Rab5, (C) UAS-YFP-Rab7, or (D) UAS-YFP-Rab11. Yellow arrowheads indicate the apical junctions in the border cell cluster. (A′–D′) Images show the single channel of aPKC in A–D. (E and F) Images of anti-aPKC and Arm co-staining border cell clusters of c306-Gal4 driven UAS-cactin-RNAi together with (E) control (cross to w1118) or (F) aPKC null mutant heterozygote. Yellow arrowheads indicate the apical junctions in the border cell cluster. (E′ and F′) Images show the single channel of aPKC in E and F. (G) Schematic of Grab-FP system in which an anti-GFP nanobody is tethered either apically or basolaterally and thereby relocalizes GFP-tagged target proteins to those domains. (H and I) Images of posterior follicle cells in egg chambers of c306-Gal4 combined with EGFP-aPKC together with (H) UAS-GrabFP-Apical or (I) UAS-GrabFP-Basal. Yellow arrowheads indicate the basolateral junctions in the follicle cells. (H′ and I′) Images show the single channel of EGFP-aPKC in H and I. (J and K) Images of anti-GFP and Arm co-staining border cell clusters of c306-Gal4 driven UAS-cactin-RNAi, UAS-GrabFP-Basal together with (J) control (cross to w1118) or (K) EGFP-aPKC heterozygote. (L and M) Images of anti-GFP and Arm co-staining border cell clusters of c306-Gal4 driven UAS-GrabFP-Apical together with (L) UAS-cactin-RNAi or (M) EGFP-aPKC heterozygote. Yellow arrowheads indicate the apical junctions in the border cell cluster. (J′–M′) Images show the single channel of anti-GFP in J–M. (N) Quantification of delamination defects in stage 10 egg chambers.

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