Excess apical Crb in Cactin knockdown cells. Related to Fig. 3. (A–B″) HS-flp-out clones showing anti-Arm staining in follicle cells. (A–A″) Control (UAS-w-RNAi). (B–B″) UAS-cactin-RNAi. (A′,A″, B′, and B″) Images show the single channels in A and B. (C) Quantification of Crb and Arm staining intensity in HS-flp-out clones. The ratio of Crb and Arm staining intensity in RNAi clones (GFP+ cells)/non-RNAi clones (GFP− cells) is shown. (D–E″) Images of anti-aPKC and anti-Arm co-staining border cell clusters with c306-Gal4 together with (D) control (cross to w1118) or (E) UAS-cactin-RNAi. (D′, D″, E′, and E″) Images show the single channels of aPKC and Arm in D and E. (F and G) Snapshots of time-lapse videos of c306-Gal4 combined with sqh-mCherry together with (F) control (cross to w1118) or (G) UAS-cactin-RNAi. Red arrowheads indicate the dynamic localization of sqh-mCherry during the delamination process. (H–I″) Images of anti-aPKC and anti-mCherry co-staining border cell clusters with c306-Gal4 combined with sqh-mCherry together with (H) control (cross to w1118) or (I) UAS-cactin-RNAi. (H′, H″, I′, and I″) Images show the single channels of aPKC and sqh-mCherry in H and I. Scale bars, 20 μm.