Figure 2.
Cactin is required for protrusion and border cell cluster polarization. (A and C) Images of anti-FasIII staining early stage 9 egg chambers with c306-Gal4 driving UAS-LifeAct-GFP together with (A) control (crossed to w1118) or (C) UAS-cactin-RNAi. Yellow arrowheads indicate the apical junctions in the border cell cluster. Red asterisks mark the polar cells. Note that FasIII stained the interface between two polar cells. (A′–A‴ and C′–C‴) Images show the single channels in A and C. (B and D) Schematic of border cell organization in A and C. To avoid confusion, the angle of the two polar cell interfaces was adjusted in B. (E and F) Snapshots of time-lapse videos of c306-Gal4 driving UAS-LifeAct-GFP together with (E) control (cross to w1118) or (F) UAS-cactin-RNAi. Scale bars, 20 μm.

Cactin is required for protrusion and border cell cluster polarization. (A and C) Images of anti-FasIII staining early stage 9 egg chambers with c306-Gal4 driving UAS-LifeAct-GFP together with (A) control (crossed to w1118) or (C) UAS-cactin-RNAi. Yellow arrowheads indicate the apical junctions in the border cell cluster. Red asterisks mark the polar cells. Note that FasIII stained the interface between two polar cells. (A′–A‴ and C′–C‴) Images show the single channels in A and C. (B and D) Schematic of border cell organization in A and C. To avoid confusion, the angle of the two polar cell interfaces was adjusted in B. (E and F) Snapshots of time-lapse videos of c306-Gal4 driving UAS-LifeAct-GFP together with (E) control (cross to w1118) or (F) UAS-cactin-RNAi. Scale bars, 20 μm.

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