Cactin functions independently of JAK/STAT or Toll signaling pathways in border cells. Related to Fig. 1. (A and B) Images of stage 8 egg chambers of c306-Gal4, ubi-Gal80ts combined with 10×STAT-GFP together with (A) control (crossed to w1118) or (B) UAS-cactin-RNAi. White asterisks mark the polar cells. (A′ and B′) 10×STAT-GFP single channel. The yellow dashed lines mark the follicle cells next to the polar cells. (C) Quantification of the 10× STAT-GFP intensity in marked follicle cells. Each dot represents an egg chamber. (D–F) Images of anti-Cactus staining with c306-Gal4 driving UAS-LifeAct-GFP together with (D) control (crossed to w1118), (E) UAS-cactus, or (F) UAS-cactin-RNAi. (D′–F′) Images show the anti-Cactus single channel. (G) Quantification of delamination defects in stage 10 egg chambers with UAS-RNAi lines of Toll signaling components. (H–K) Images of anti-Dorsal staining with c306-Gal4 driving UAS-LifeAct-GFP together with (H) control (crossed to w1118) early stages, (I) control (crossed to w1118) stage 9, (J) UAS-cactin-RNAi, stage 9, or (K) UAS-dorsal-RNAi, stage 9. (H′–K′) Images show the anti-Dorsal single channel. (H) Inset shows the high magnification image of the stalk. White arrowheads indicate the stalks. Yellow arrowheads indicate the polar cells. Scale bars, 20 μm.