Multiple cactin-RNAi lines cause delamination defects. Related to Fig. 1. (A–D) Confocal images of stage 10 egg chambers with c306-Gal4 driving UAS-LifeAct-GFP together with (A) control (crossed to w1118), (B) UAS-cactin-RNAi (v32718GD), (C) UAS-cactin-RNAi (v32719GD), or (D) UAS-cactin-RNAi (v106976KK). Insets show high magnification of border cell clusters. Yellow arrowheads indicate the border cell cluster position. (E and F) HS-flp-out clones showing UAS-cactin-RNAi efficiency in follicle cells. GFP (green) labels Gal4-expressing clones. Hoechst DNA dye is in blue. Cactin::RFP is in magenta. (E) Image of UAS-cactin::RFP with UAS-w-RNAi. (F) Image of UAS-cactin::RFP with UAS-cactin-RNAi. Yellow arrowheads indicate the Cactin-RFP expression in clones. (E′ and F′) Cactin-RFP single channel in E and F. (F′) Imaging settings were the same for E′ and F′, whereas the inset in F′ shows enhanced contrast to reveal the low level of remaining Cactin-RFP expression. Scale bars, 20 μm. (G) Quantification of Cactin-RFP intensity in HS-flp-out clone cells. The mean of control (w-RNAi) is normalized to 1. Each dot represents a cell.