Figure 1.
Cactin is required for border cell delamination. (A–D) Confocal micrographs of c306-Gal4, UAS-LifeAct-GFP/+ egg chambers of the indicated stages. differential interference contrast imaging is shown in grayscale, DNA in blue, and GFP in green. (A′–D′) High-magnification images of the GFP channels for A–D. (E–H) Images of similar stage egg chambers from c306-Gal4, UAS-LifeAct-GFP/cactin-RNAi females, labeled as in A–D. (E′–H′) High magnification images of E–H. (I–K) Schematic of the expression patterns of (I) c306-Gal4, (J) fruitless-Gal4, and (K) upd-Gal4. (L) Quantification of delamination defects in stage 10 egg chambers. Histogram shows the distribution of border cell clusters along the migration path in stage 10. (M–O) Confocal images of stage 10 egg chambers with c306-Gal4 driving UAS-cactin-RNAi together with (M) UAS-mCherry-CAAX, (N) UAS-cactin, or (O) UAS-cactin::RFP in magenta. Yellow arrowheads indicate the border cell clusters. Scale bars, 20 μm.

Cactin is required for border cell delamination. (A–D) Confocal micrographs of c306-Gal4, UAS-LifeAct-GFP/+ egg chambers of the indicated stages. differential interference contrast imaging is shown in grayscale, DNA in blue, and GFP in green. (A′–D′) High-magnification images of the GFP channels for A–D. (E–H) Images of similar stage egg chambers from c306-Gal4, UAS-LifeAct-GFP/cactin-RNAi females, labeled as in A–D. (E′–H′) High magnification images of E–H. (I–K) Schematic of the expression patterns of (I) c306-Gal4, (J) fruitless-Gal4, and (K) upd-Gal4. (L) Quantification of delamination defects in stage 10 egg chambers. Histogram shows the distribution of border cell clusters along the migration path in stage 10. (M–O) Confocal images of stage 10 egg chambers with c306-Gal4 driving UAS-cactin-RNAi together with (M) UAS-mCherry-CAAX, (N) UAS-cactin, or (O) UAS-cactin::RFP in magenta. Yellow arrowheads indicate the border cell clusters. Scale bars, 20 μm.

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