Figure 5.
Localization of RPB3, H2B, PCNA, CDK9, and CDK12 with respect to RNAP2 Ser2ph-mintbody. HeLa cells stably expressing RNAP2 Ser2ph-mintbody were transfected with expression vectors for HaloTag-tagged proteins and stained with 50 nM HaloTag TMR ligand for 30 min before live-cell imaging using a high-resolution confocal system. The averaged images of 10 consecutive frames (551 ms/frame) are shown with magnified views of areas indicated in orange (middle three rows). Line intensity profiles of 4-µm lines in the second row are shown (fifth row) by normalizing against the average nuclear intensity to yield relative intensity ratios. The CCFs of 10 cells are shown at the bottom (blue, average; orange, individual cells). Scale bars, 5 µm; 1 µm for magnified views.

Localization of RPB3, H2B, PCNA, CDK9, and CDK12 with respect to RNAP2 Ser2ph-mintbody. HeLa cells stably expressing RNAP2 Ser2ph-mintbody were transfected with expression vectors for HaloTag-tagged proteins and stained with 50 nM HaloTag TMR ligand for 30 min before live-cell imaging using a high-resolution confocal system. The averaged images of 10 consecutive frames (551 ms/frame) are shown with magnified views of areas indicated in orange (middle three rows). Line intensity profiles of 4-µm lines in the second row are shown (fifth row) by normalizing against the average nuclear intensity to yield relative intensity ratios. The CCFs of 10 cells are shown at the bottom (blue, average; orange, individual cells). Scale bars, 5 µm; 1 µm for magnified views.

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