Figure 4.
Surf4 is required for the efficient exit of prosaposin from the ER.(A and B) Live-cell imaging of prosaposin-RUSH traffic from ER in control and Surf4 KO cells at 0-, 15-, and 30-min time points after biotin addition; scale bar, 10 µm. Magnified insets show the localization of prosaposin to GFP-GalT–labeled Golgi; inset scale bar, 2 µm. (C) Quantification of the delivery of prosaposin-RUSH to the Golgi in control and Surf4 KO cells. The mean fluorescence intensities of prosaposin-RUSH localized to the Golgi (defined by GalT-GFP signal) after biotin addition were normalized to the mean fluorescence intensities before biotin (baseline marked by gray line) and plotted at the indicated times of biotin treatment. Data were collected from four independent experiments with n = 9–14 cells per experiment; error bars show mean ± SEM.

Surf4 is required for the efficient exit of prosaposin from the ER. (A and B) Live-cell imaging of prosaposin-RUSH traffic from ER in control and Surf4 KO cells at 0-, 15-, and 30-min time points after biotin addition; scale bar, 10 µm. Magnified insets show the localization of prosaposin to GFP-GalT–labeled Golgi; inset scale bar, 2 µm. (C) Quantification of the delivery of prosaposin-RUSH to the Golgi in control and Surf4 KO cells. The mean fluorescence intensities of prosaposin-RUSH localized to the Golgi (defined by GalT-GFP signal) after biotin addition were normalized to the mean fluorescence intensities before biotin (baseline marked by gray line) and plotted at the indicated times of biotin treatment. Data were collected from four independent experiments with n = 9–14 cells per experiment; error bars show mean ± SEM.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal