Figure 3.
Surf4 is required for the trafficking of both progranulin and prosaposin.(A) Confocal images of progranulin and prosaposin in control and Surf4 KO cells along with LAMP1-labeled lysosomes. Scale bar, 10 µm; inset, 3.38 µm wide. (B) Confocal images of progranulin and calnexin (ER protein) in control and Surf4 KO cells. Scale bar, 10 µm; inset, 3.38 µm wide. (C) Immunoblot analysis for progranulin in control versus Surf4 KO cell lysates without (−) or with (+) Endo H treatment. (D–F) Quantification of immunoblots in C. (G) Immunoblot analysis for prosaposin in control versus Surf4 KO lysates. S, Endo H sensitive; R, Endo H resistant. (H–J) Quantification of immunoblots in G. In C–J, n = 4 independent experiments; mean ± SEM; unpaired t test; **, P < 0.01; ***, P < 0.001.

Surf4 is required for the trafficking of both progranulin and prosaposin. (A) Confocal images of progranulin and prosaposin in control and Surf4 KO cells along with LAMP1-labeled lysosomes. Scale bar, 10 µm; inset, 3.38 µm wide. (B) Confocal images of progranulin and calnexin (ER protein) in control and Surf4 KO cells. Scale bar, 10 µm; inset, 3.38 µm wide. (C) Immunoblot analysis for progranulin in control versus Surf4 KO cell lysates without (−) or with (+) Endo H treatment. (D–F) Quantification of immunoblots in C. (G) Immunoblot analysis for prosaposin in control versus Surf4 KO lysates. S, Endo H sensitive; R, Endo H resistant. (H–J) Quantification of immunoblots in G. In C–J, n = 4 independent experiments; mean ± SEM; unpaired t test; **, P < 0.01; ***, P < 0.001.

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