Figure S4.
CLN6 and ERGIC-53 are not required for the localization of progranulin to lysosomes.(A) Confocal immunofluorescence images showing progranulin localization to LAMP1-positive late endosomes and lysosomes in embryonic fibroblasts from WT and homozygous CLN6 mutant MEFs. Scale bar, 10 µm; inset, 3.38 µm wide. (B) Immunoblot shows the ERGIC-53 protein levels in control and ERGIC-53 siRNA–transfected cells. (C) Quantification of immunoblots where the graph shows ERGIC-53 levels normalized to loading control (n = 3 independent experiments; mean ± SEM; unpaired t test; ****, P < 0.0001). (D) Immunofluorescence images showing progranulin and prosaposin localization similar to LAMP1-labeled endosomes/lysosomes in control and ERGIC-53 KD cells. Scale bar, 10 µm; inset, 5 µm wide.

CLN6 and ERGIC-53 are not required for the localization of progranulin to lysosomes. (A) Confocal immunofluorescence images showing progranulin localization to LAMP1-positive late endosomes and lysosomes in embryonic fibroblasts from WT and homozygous CLN6 mutant MEFs. Scale bar, 10 µm; inset, 3.38 µm wide. (B) Immunoblot shows the ERGIC-53 protein levels in control and ERGIC-53 siRNA–transfected cells. (C) Quantification of immunoblots where the graph shows ERGIC-53 levels normalized to loading control (n = 3 independent experiments; mean ± SEM; unpaired t test; ****, P < 0.0001). (D) Immunofluorescence images showing progranulin and prosaposin localization similar to LAMP1-labeled endosomes/lysosomes in control and ERGIC-53 KD cells. Scale bar, 10 µm; inset, 5 µm wide.

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