Full-length progranulin is predominantly sensitive to Endo H. (A) Immunoblot analysis of Endo H sensitivity of the endogenous progranulin in cell lysates collected from control HeLa cells. (B) Blot quantification shows the percentage of progranulin forms present in control cells. n = 3 experiments; mean ± SEM. (C) Immunoblot showing the levels of stably expressed mCherry-progranulin in comparison to endogenous progranulin levels in HeLa cells. (D) Immunoblot analysis of mCherry-progranulin forms upon Endo H digestion of cell lysates collected from HeLa cells stably expressing mCherry-progranulin. (E) Immunoblot quantification showing the percentage of mCherry-progranulin forms present. n = 4 experiments; mean ± SEM.