Additional early Golgi proteins resemble Vrg4 in their kinetic signatures. (A) Maturation kinetics of Drs2 compared with Anp1. A strain expressing HaloTag-Drs2 and the mannosyltransferase Anp1-GFP was grown to mid-log phase, labeled with JFX dye, and imaged by 4D confocal microscopy. Shown are average projected Z-stacks at the indicated time points from part 2 of Video 5. The upper row shows the complete projections, the second row shows edited projections that include only the cisterna being tracked, and the subsequent rows show the individual fluorescence channels from the edited projections. Scale bar, 2 µm. (B) Quantification of tagged Golgi proteins during a typical maturation event. Depicted are the normalized fluorescence intensities in arbitrary units for the cisterna tracked in A. (C) Smoothed and averaged traces showing the relative kinetic signatures of Drs2 and Anp1. Data were obtained for 10 representative cisternae. (D) Maturation kinetics of Drs2 compared with Mnn9. The experiment was performed as in A, except that the strain expressed the mannosyltransferase Mnn9-GFP, and the images are from part 3 of Video 5. Scale bar, 2 µm. (E) Quantification of tagged Golgi proteins during a typical maturation event. Depicted are the normalized fluorescence intensities in arbitrary units for the cisterna tracked in D. (F) Smoothed and averaged traces showing the relative kinetic signatures of Drs2 and Mnn9. Data were obtained for 13 representative cisternae.