CK-666 affects the localizations of transmembrane TGN proteins but not of transmembrane early Golgi proteins in double mutant cells lacking both AP-1 and Ent5. (A and B) This figure extends Fig. 3 E, with the inclusion of additional marker proteins and of data from apl4Δ ENT5 and APL4 ent5Δ cells. (A) Quantification of the CK-666 effects for five transmembrane TGN proteins in wild-type cells and in cells lacking either AP-1 or Ent5 or both. (B) Quantification of the CK-666 effects for three transmembrane early Golgi proteins in wild-type cells and in cells lacking either AP-1 or Ent5 or both. Error bars represent SEM.