CK-666 affects the localizations of some transmembrane TGN proteins in single mutant cells lacking either AP-1 or Ent5. (A) Effect of CK-666 on the localizations of transmembrane TGN proteins in the absence of AP-1. The experiment was performed as in Fig. 3 D, except that apl4Δ ENT5 cells were used. CK-666 caused mislocalization of Drs2 but not of Tlg1, Kex2, or Vrg4. Scale bar, 2 µm. (B) Effect of CK-666 on the localizations of transmembrane TGN proteins in the absence of Ent5. The experiment was performed as in Fig. 3 D, except that APL4 ent5Δ cells were used. CK-666 caused mislocalization of Drs2 and Tlg1 but not of Kex2 or Vrg4. Scale bar, 2 µm.