CK-666 does not affect the localizations of transmembrane Golgi proteins in wild-type cells. (A) Lack of a CK-666 effect on the localizations of transmembrane TGN proteins. The experiment was performed as in Fig. 3 D, except that APL4 ENT5 cells were used and GFP-Ste13 and Stv1-GFP data are also shown. Scale bar, 2 µm. (B) Lack of a CK-666 effect on the localizations of transmembrane early Golgi proteins. The experiment was performed as in Fig. 3 D, except that APL4 ENT5 cells were used and Anp1-GFP and Mnn9-GFP data are also shown. Scale bar, 2 µm.