Figure 7.
Nuclear accumulation of the transcriptional repressor Xbp1 promotes high-Cdk1 quiescence. (A) Schematic copper/starvation–induction protocol to assess the effect of increased Xbp1 levels during starvation. (B) Percentage of high-Cdk1 Q-cells after CUP1p-expression of mTFP1 C-terminally tagged WT Xbp1 (n = 6; OAM458), the DNA-binding domain mutant xbp1-EE (n = 7; OAM466), the Xbp1-related transcriptional repressor Stb3 (n = 4; OAM497), or the cell cycle repressor Whi5 (n = 7; OAM455) during starvation. Ctrl, isogenic control strain (OAM454). (C) Nuclear accumulation of endogenously expressed Xbp1-mNeonGreen in cells expressing mTFP1 C-terminally tagged wild-type Xbp1 (n = 6) or the DNA-binding domain mutant xbp1-EE (n = 7). (D) Final nuclear intensity of endogenously expressed Xbp1-mNeonGreen in cells expressing mTFP1–C-terminally tagged Xbp1 (n = 6), xbp1-EE (n = 7), Stb3 (n = 4), or Whi5 (n = 7) from the CUP1 promoter during starvation. (E and F) Single-cell time series heatmaps contrasting the opposing pattern of Cdc10-mCyOFP1 (E) and nuclear Whi5-mTFP1 (F) after CUP1p-expression of Whi5-mTFP1 during starvation. (G and H) Average nuclear intensity of CUP1p-expressed Whi5-mTFP1 (G; n = 7) or Xbp1-mTFP1 (H; n = 4) in low-Cdk1 (blue) and high-Cdk1 (red) Q-cells. (I and J) Percentage of high-Cdk1 Q-cells (I) and their viability (J), as measured by proliferation upon return to rich medium after 20 h of starvation, in xbp1Δ (n = 4; 500 cells, OAM500) and control XBP1 cells (Ctrl; n = 4; 311 cells, OAM502). Red arrowheads = onset of starvation. Solid lines with shaded area = average ± 95% confidence intervals. Red star = P < 0.05, K-S test. Bar plots = mean ± SD.

Nuclear accumulation of the transcriptional repressor Xbp1 promotes high-Cdk1 quiescence. (A) Schematic copper/starvation–induction protocol to assess the effect of increased Xbp1 levels during starvation. (B) Percentage of high-Cdk1 Q-cells after CUP1p-expression of mTFP1 C-terminally tagged WT Xbp1 (n = 6; OAM458), the DNA-binding domain mutant xbp1-EE (n = 7; OAM466), the Xbp1-related transcriptional repressor Stb3 (n = 4; OAM497), or the cell cycle repressor Whi5 (n = 7; OAM455) during starvation. Ctrl, isogenic control strain (OAM454). (C) Nuclear accumulation of endogenously expressed Xbp1-mNeonGreen in cells expressing mTFP1 C-terminally tagged wild-type Xbp1 (n = 6) or the DNA-binding domain mutant xbp1-EE (n = 7). (D) Final nuclear intensity of endogenously expressed Xbp1-mNeonGreen in cells expressing mTFP1–C-terminally tagged Xbp1 (n = 6), xbp1-EE (n = 7), Stb3 (n = 4), or Whi5 (n = 7) from the CUP1 promoter during starvation. (E and F) Single-cell time series heatmaps contrasting the opposing pattern of Cdc10-mCyOFP1 (E) and nuclear Whi5-mTFP1 (F) after CUP1p-expression of Whi5-mTFP1 during starvation. (G and H) Average nuclear intensity of CUP1p-expressed Whi5-mTFP1 (G; n = 7) or Xbp1-mTFP1 (H; n = 4) in low-Cdk1 (blue) and high-Cdk1 (red) Q-cells. (I and J) Percentage of high-Cdk1 Q-cells (I) and their viability (J), as measured by proliferation upon return to rich medium after 20 h of starvation, in xbp1Δ (n = 4; 500 cells, OAM500) and control XBP1 cells (Ctrl; n = 4; 311 cells, OAM502). Red arrowheads = onset of starvation. Solid lines with shaded area = average ± 95% confidence intervals. Red star = P < 0.05, K-S test. Bar plots = mean ± SD.

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