Figure 6. Nuclear accumulation of... | Rockefeller University Press

Figure 6.

$Nuclear accumulation of stress-activated transcription factors predicts high-Cdk1 quiescence entry depending on starvation conditions.(A) Schematic quantification of cluster discrimination by an LDC based on the Mahalanobis distance. (B and C) Average cluster separation between low-Cdk1 (blue) and high-Cdk1 (red) Q-cells, as measured by the Mahalanobis distance, calculated using LDCs based on the combined nuclear intensity values of Xbp1 and Gln3 (B) or all stress markers (C). (D) Probabilities of high-Cdk1 quiescence during starvation in a representative high-Cdk1 (left) and low-Cdk1 (right) Q-cell as measured by an SVM approach. (E–G) Average probability of high-Cdk1 quiescence assigned to high-Cdk1 Q-cells by SVMs based on the final nuclear intensity of Gln3 after exposure to rich medium (E), glucose depletion (F), or nitrogen depletion (G) before starvation. (H–J) Average probability of high-Cdk1 quiescence assigned to high-Cdk1 Q-cells by SVMs based on the final nuclear Xbp1 intensity after exposure to rich medium (H), glucose depletion (I), or nitrogen depletion (J) before starvation. Red arrowhead with horizontal dotted line = a priori probability threshold corresponding to the fraction of high-Cdk1 Q-cells in each experiment. Solid lines with shaded area = average ± 95% confidence intervals based on biological replicates (n > 3).$

Nuclear accumulation of stress-activated transcription factors predicts high-Cdk1 quiescence entry depending on starvation conditions. (A) Schematic quantification of cluster discrimination by an LDC based on the Mahalanobis distance. (B and C) Average cluster separation between low-Cdk1 (blue) and high-Cdk1 (red) Q-cells, as measured by the Mahalanobis distance, calculated using LDCs based on the combined nuclear intensity values of Xbp1 and Gln3 (B) or all stress markers (C). (D) Probabilities of high-Cdk1 quiescence during starvation in a representative high-Cdk1 (left) and low-Cdk1 (right) Q-cell as measured by an SVM approach. (E–G) Average probability of high-Cdk1 quiescence assigned to high-Cdk1 Q-cells by SVMs based on the final nuclear intensity of Gln3 after exposure to rich medium (E), glucose depletion (F), or nitrogen depletion (G) before starvation. (H–J) Average probability of high-Cdk1 quiescence assigned to high-Cdk1 Q-cells by SVMs based on the final nuclear Xbp1 intensity after exposure to rich medium (H), glucose depletion (I), or nitrogen depletion (J) before starvation. Red arrowhead with horizontal dotted line = a priori probability threshold corresponding to the fraction of high-Cdk1 Q-cells in each experiment. Solid lines with shaded area = average ± 95% confidence intervals based on biological replicates (n > 3).