Figure 4.
Low- and high-Cdk1 quiescent states share a core of stress-associated processes. (A–D) Quantification of nonmetabolic stress responses in low-Cdk1 (blue) or high-Cdk1 (red) Q-cells upon starvation (n = 3; 255 cells, OAM667). (A) Average nuclear intensity of the general stress transcription factor Msn2-mNeonGreen per cluster. (B) Average nuclear intensity of the calcium stress transcription factor Crz1-mTFP1 per cluster. (C) Average nuclear intensity of the osmotic stress kinase Hog1-mNeptune2.5 per cluster. (D) Average intensity (int.) of the cell wall stress kinase Pkc1-mRuby3 at cell membrane per cluster. (E) Representative micrographs of cells bearing the autophagy reporter mNeonGreen-Atg8 upon starvation. MIP mNeonGreen image superimposed on phase contrast. (F) Average number of mNeonGreen-Atg8 foci per cluster upon starvation (n = 3; 450 cells, OAM654). (G) Representative micrographs of cells bearing the protein aggregation sensor QN-mRuby3 upon starvation. MIP fluorescent mRuby3 image superimposed on phase contrast. (H) Average number of QN-mRuby3 foci per cluster upon starvation (n = 3; 333 cells, OAM671). (I) Representative micrographs of cells bearing the mitochondrial marker Tom70-mTFP1 upon starvation. MIP mTFP1 image superimposed on phase contrast. (J) Average total intensity of Tom70-mTFP1 per cluster upon starvation (n = 3; 225 cells, OAM673). (K) Representative micrographs of cells bearing the DNA damage marker Rad52-mNeonGreen upon starvation. MIP mNeonGreen image superimposed on phase contrast. (L) Average Rad52-mNeonGreen nuclear intensity per cluster upon starvation (n = 3; 240 cells, OAM670). Solid lines with shaded area = average ± 95% confidence intervals. Red arrowheads = onset of starvation.

Low- and high-Cdk1 quiescent states share a core of stress-associated processes. (A–D) Quantification of nonmetabolic stress responses in low-Cdk1 (blue) or high-Cdk1 (red) Q-cells upon starvation (n = 3; 255 cells, OAM667). (A) Average nuclear intensity of the general stress transcription factor Msn2-mNeonGreen per cluster. (B) Average nuclear intensity of the calcium stress transcription factor Crz1-mTFP1 per cluster. (C) Average nuclear intensity of the osmotic stress kinase Hog1-mNeptune2.5 per cluster. (D) Average intensity (int.) of the cell wall stress kinase Pkc1-mRuby3 at cell membrane per cluster. (E) Representative micrographs of cells bearing the autophagy reporter mNeonGreen-Atg8 upon starvation. MIP mNeonGreen image superimposed on phase contrast. (F) Average number of mNeonGreen-Atg8 foci per cluster upon starvation (n = 3; 450 cells, OAM654). (G) Representative micrographs of cells bearing the protein aggregation sensor QN-mRuby3 upon starvation. MIP fluorescent mRuby3 image superimposed on phase contrast. (H) Average number of QN-mRuby3 foci per cluster upon starvation (n = 3; 333 cells, OAM671). (I) Representative micrographs of cells bearing the mitochondrial marker Tom70-mTFP1 upon starvation. MIP mTFP1 image superimposed on phase contrast. (J) Average total intensity of Tom70-mTFP1 per cluster upon starvation (n = 3; 225 cells, OAM673). (K) Representative micrographs of cells bearing the DNA damage marker Rad52-mNeonGreen upon starvation. MIP mNeonGreen image superimposed on phase contrast. (L) Average Rad52-mNeonGreen nuclear intensity per cluster upon starvation (n = 3; 240 cells, OAM670). Solid lines with shaded area = average ± 95% confidence intervals. Red arrowheads = onset of starvation.

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