Figure 8.
Accumulation of M6PR in endosomes in ORP10 KO cells.(A) Immunofluorescence staining of endogenous CI-M6PR and EEA1 in control (Cont), ORP10 KO cells, ORP10 KO cells rescued by GFP fused to ORP10 (ORP10Full), ΔORD (ORP10ΔORD), or ORP9. Scale bars, 1 µm (full size) and 1 µm (inset). (B) Colocalization analysis of A (mean ± SEM; n ≥ 20 cells). ****, P < 0.0001.

Accumulation of M6PR in endosomes in ORP10 KO cells. (A) Immunofluorescence staining of endogenous CI-M6PR and EEA1 in control (Cont), ORP10 KO cells, ORP10 KO cells rescued by GFP fused to ORP10 (ORP10Full), ΔORD (ORP10ΔORD), or ORP9. Scale bars, 1 µm (full size) and 1 µm (inset). (B) Colocalization analysis of A (mean ± SEM; n ≥ 20 cells). ****, P < 0.0001.

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