Figure 6.
M6PR retrograde trafficking to TGN is delayed in ORP10 KO cells. Left: Control (Cont) or ORP10 KO cells expressing Tac-M6PR were incubated with anti-Tac antibody for 60 min to monitor retrograde trafficking to the TGN, followed by immunofluorescence staining with golgin 97 antibody. Right: Graph shows quantification of Tac-M6PR immunofluorescence ratio (vesicular/Golgi; mean ± SEM, n ≥ 6). Scale bars, 10 µm (full size) and 2 µm (inset). **, P < 0.01.

M6PR retrograde trafficking to TGN is delayed in ORP10 KO cells. Left: Control (Cont) or ORP10 KO cells expressing Tac-M6PR were incubated with anti-Tac antibody for 60 min to monitor retrograde trafficking to the TGN, followed by immunofluorescence staining with golgin 97 antibody. Right: Graph shows quantification of Tac-M6PR immunofluorescence ratio (vesicular/Golgi; mean ± SEM, n ≥ 6). Scale bars, 10 µm (full size) and 2 µm (inset). **, P < 0.01.

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