Figure S3.
Acute recruitment of ORDORP10 to ER–endosome MCSs.(A and B) Live-cell confocal microscopic images showing acute recruitment of mScarlet-FKBP-ORDORP10 from cytosol (0 min) to the ER–endosome MCSs tethered by ORP9-ΔORD-FRB-iRFP (5 min) upon addition of 250 nM rapamycin (Rapa.; A) and its kinetics (B; mean ± SEM; n = 11 cells). Scale bar, 5 µm. (C and D) Representative snapshot images of confocal live microscopy showing PI4P levels (GFP-OSBP-PH; upper) or PS levels (mRFP-LactC2; lower) before (0 min) and after (5 min) acute recruitment of ORDORP10-HHAA to ER–endosome MCSs (C) and its quantification (D; mean ± SEM; n = 11 cells). Scale bars, 5 µm (full size) and 1 µm (inset).

Acute recruitment of ORDORP10 to ER–endosome MCSs. (A and B) Live-cell confocal microscopic images showing acute recruitment of mScarlet-FKBP-ORDORP10 from cytosol (0 min) to the ER–endosome MCSs tethered by ORP9-ΔORD-FRB-iRFP (5 min) upon addition of 250 nM rapamycin (Rapa.; A) and its kinetics (B; mean ± SEM; n = 11 cells). Scale bar, 5 µm. (C and D) Representative snapshot images of confocal live microscopy showing PI4P levels (GFP-OSBP-PH; upper) or PS levels (mRFP-LactC2; lower) before (0 min) and after (5 min) acute recruitment of ORDORP10-HHAA to ER–endosome MCSs (C) and its quantification (D; mean ± SEM; n = 11 cells). Scale bars, 5 µm (full size) and 1 µm (inset).

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