Figure S2. ORD ORP10 ligand and its... | Rockefeller University Press

Figure S2.

ORDORP10 ligand and its lipid countertransport activity.(A) Phosphoinositide species in Expi293F cells (107 cells) detected by MS (mean ± SEM; n = 3 independent experiments). (B) Coomassie Brilliant Blue–stained SDS-PAGE gel of ORDORP8 proteins purified from E. coli. (C) Kinetic curves of transport of PS (µM) to acceptor liposomes doped with only PC (gray) or PC + PI4P (red) by ORDORP8. (D) Kinetic curves of transport of PI4P (µM) to acceptor liposomes doped with only PC (gray) or PC + PS (blue) by ORDORP8. (E) Coomassie Brilliant Blue–stained SDS-PAGE gel of ORDORP10 proteins purified from E. coli. (F) Kinetic curves of transport of PS (µM) to acceptor liposomes doped with only PC (gray) or PC + PI4P (red) by ORDORP10. (G) Kinetic curves of transport of PI4P (µM) to acceptor liposomes doped with only PC (gray) or PC + PS (blue) by ORDORP10 (mean ± SD; n = 3 independent experiments).

ORD^ORP10 ligand and its lipid countertransport activity. (A) Phosphoinositide species in Expi293F cells (10⁷ cells) detected by MS (mean ± SEM; n = 3 independent experiments). (B) Coomassie Brilliant Blue–stained SDS-PAGE gel of ORD^ORP8 proteins purified from E. coli. (C) Kinetic curves of transport of PS (µM) to acceptor liposomes doped with only PC (gray) or PC + PI4P (red) by ORD^ORP8. (D) Kinetic curves of transport of PI4P (µM) to acceptor liposomes doped with only PC (gray) or PC + PS (blue) by ORD^ORP8. (E) Coomassie Brilliant Blue–stained SDS-PAGE gel of ORD^ORP10 proteins purified from E. coli. (F) Kinetic curves of transport of PS (µM) to acceptor liposomes doped with only PC (gray) or PC + PI4P (red) by ORD^ORP10. (G) Kinetic curves of transport of PI4P (µM) to acceptor liposomes doped with only PC (gray) or PC + PS (blue) by ORD^ORP10 (mean ± SD; n = 3 independent experiments).