Complete loss of dynein-2 motility results in ciliary accumulations even when the TZ barrier is completely disrupted. (A, C, E, and G) Representative examples of phasmid cilia of the indicated TZ mutant genotypes, expressing wild-type GFP::CHE-3 or the nonmotile GFP::CHE-3(K2935Q). (B, D, F, and H) Relative distribution of GFP::CHE-3(K2935Q) signal intensity along cilia, in control and TZ mutant backgrounds. The gray dashed line represents the data from the GFP::CHE-3(K2935Q) mutant in B. Gray rectangles highlight the TZ, as previously defined. n ≥ 105 cilia for B, n ≥ 104 cilia for D, n ≥ 80 cilia for F, and n ≥ 86 cilia for H. XY intensity distribution graphs are shown as mean ± SEM. (I) Length of GFP::CHE-3(K2935Q) mutant cilia analyzed in B, D, F, and H, with the same color-coded genotypes as indicated. Graph is shown as mean ± SD. Kruskal–Wallis test followed by Dunn’s multiple comparison were used to analyze these datasets. *, P ≤ 0.05; **, P ≤ 0.01. (J) Cilia and the respective kymographs from the specified strain genotypes. No anterograde or retrograde IFT was detectable in the GFP::CHE-3(K2935Q) mutant, not even in combination with the disruption of MKS-5 or NPHP-4. Scale bars: 2 µm (A, C, E, and G); vertical 5 s, horizontal 2 µm (J).