Dynein-2 accumulates on the distal side of the TZ, unable to complete retrograde IFT in wdr-60 mutants. (A) GFP::CHE-3 localization relative to the centriolar wall component mCherry::HYLS-1 at the base of phasmid cilia of the indicated strains. (B) GFP::CHE-3 localization relative to the MKS-6::mCherry TZ component in phasmid cilia of the indicated strains. (C) Quantification GFP::CHE-3 signal distribution in relation to the TZ, as determined by MKS-6 localization (n ≥ 38 cilia). Gray rectangles define the TZ region. 3× magnifications of the square section in each micrograph were included to better visualize the distribution of the dynein-2 HC relative to the base and TZ of wdr-60 mutant cilia. Graphs are shown as mean ± SEM. Scale bars: 2 µm.